This Attah_readme20230207.txt file was generated on 20230207 by Reuben Attah (Please update the file name to read AUTHOR_readmeYYYYMMDD.txt) ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset: Dataset for: The effects of photochemical aging and interactions with secondary organic aerosols on cellular toxicity of combustion particles 2. Author Information Principal Investigator Contact Information Name: Kerry Kelly Institution: University of Utah Address:2288 MEB, University of Utah Email: Kerry.kelly@utah.edu Associate or Co-investigator Contact Information Name: Reuben Attah Institution: University of Utah Address:MEB 2509 Univeristy of Utah Email: reuben.attah@utah.edu 3. Date of data collection (single date, range, approximate date) 20210601-20221231 4. Geographic location of data collection (where was data collected?): SALT LAKE CITY, UT, USA 5. Information about funding sources that supported the collection of the data: Research is supported by the National Institute of Environmental Health Sciences of the National Institutes of Health (5K25ES027504Ð02) -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: CC BY-Allows others to use and share your data, even commercially, with attribution 2. Links to publications that cite or use the data: Submitted and not published yet 3. Links to other publicly accessible locations of the data: NA 4. Links/relationships to ancillary data sets:NA 5. Was data derived from another source? If yes, list source(s): No 6. Recommended citation for the data: Kelly K, Attah R. (2023). Dataset for The effects of photochemical aging and interactions with secondary organic aerosols on cellular toxicity of combustion particles. The Hive: University of Utah Research Data Repository. Version. https://doi.org/10.7278/S50d-2a2h-jeaa --------------------- DATA & FILE OVERVIEW --------------------- 1. File A. Filename: Data_set_for_Reuben_Aging_2023.xlsx Short description: Contains sheet labeled based on the type of test (e.g EC_OC, FTIR, etc). The sheet Organic composition contains the PAH and other organic compounds, EC_OC contains elemental and organic carbon, FTIR contains surface composition, SMPS contains particle size distribution, IL8, and CYP-1A1 contains IL8 and CYP-1A1 induction for both A549 cells, and A549-THP-1 cells. B. Filename: Short description: C. Filename: Short description: 2. Relationship between files: 3. Additional related data collected that was not included in the current data package: 4. Are there multiple versions of the dataset? yes/no: No If yes, list versions: Name of file that was updated: i. Why was the file updated? ii. When was the file updated? Name of file that was updated: i. Why was the file updated? ii. When was the file updated? -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: The data was collected using experimental characterization techniques such as Scanning Mobility Particle Sizer (SMPS), Fourier transform infrared spectroscopy (FTIR), elemental and organic carbon analyzer and biological assays such as ELISA, oxidative potential and CYP1A1 induction by PCR tests. * 2. Methods for processing the data: The file contained raw data and processed data in excel form. Calculations such as averages, standard deviation, and one way analysis of variance were done suing Microsoft excel. 3. Instrument- or software-specific information needed to interpret the data: Microsoft Excel version 16.69.1(23011600),SMPS data were generated using the Aerosol Instrument Manager software version 8.2. 4. Standards and calibration information, if appropriate:None 5. Environmental/experimental conditions: Ambient conditions 6. Describe any quality-assurance procedures performed on the data: All experiments were performed in triplicate and results The results are presented as the standard error of the mean (standard deviation of the mean divided by the square root of the sample size)between the three measurements. 7. People involved with sample collection, processing, analysis and/or submission: Reuben Attah, Kamaljeet Kaur, Christopher A. Reilly, Cassandra E Deering-Rice, and Kerry Kelly. ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: [Data_set_for_Reuben_Aging_2023] ----------------------------------------- Data_set_for_Reuben_Aging_2023 Sheet: Organic composition 1. Number of variables: 3 2. Number of cases/rows: 102 3. Variable List A. Name: Fresh CPs Description: Composition of organic compound in fresh combustion particles measured in mg/Kg B. Name: Aged CPs Description: Composition of organic compound in aged combustion particles measured in mg/Kg C. Name: Aged CPs + SOA Description: Composition of organic compound in aged combustion particles coated with SOA measured in mg/Kg Sheet: EC_OC 1. Number of variables: 4 2. Number of cases/rows: 27 3. Variable List A. Name: Blank Description: Composition of Elemental carbon (EC) and organic carbon (OC) in fresh combustion particles measured in ug/sqcm B. Name: Fresh soot Description: Composition of organic compound in fresh combustion particles measured in mg/Kg C. Name: Aged soot Description: Composition of organic compound in aged combustion particles measured in mg/Kg D. Name: Aged soot + SOA Description: Composition of organic compound in aged combustion particles coated with SOA measured in mg/Kg Sheet: FTIR 1. Number of variables: 7 2. Number of cases/rows: 403 3. Variable List A. Name: Wave number Description: Wave number of the FTIR spectra measured in /cm B. Name: Kubelka-Munk_Fresh Description: Absorbance of fresh combustion particles measured by the FTIR in kubelka-munk unit C. Name: Normalized_Fresh Description: Absorbance of fresh combustion particles measured by the FTIR in the kubelka-munk unit normalized by the highest value D. Name: Kubelka-Munk_Aged Description: Absorbance of aged combustion particles measured by the FTIR in kubelka-munk unit E. Name: Normalized_Aged Description: Absorbance of aged combustion particles measured by the FTIR in the kubelka-munk unit normalized by the highest value F. Name: Kubelka-Munk_SOA Description: Absorbance of aged + SOA combustion particles measured by the FTIR in kubelka-munk unit G. Name: Normalized_SOA Description: Absorbance of aged + SOA combustion particles measured by the FTIR in the kubelka-munk unit normalized by the highest value Sheet: SMPS_particle size distribution 1. Number of variables: 7 2. Number of cases/rows:99 3. Variable List A. Name: Diameter Mid Description: Mobility diameter measured by the SMPS in nm B. Name: Fresh_Average Description: Average number of concentration of fresh combustion particles measured in #/cm3 C. Name: Fresh_SD Description: Standard deviation number of concentration of fresh combustion particles measured in #/cm3 D. Name: Aged_Average Description: Average number of concentration of aged combustion particles measured in #/cm3 E. Name: Aged_SD Description: Standard deviation number of concentration of aged combustion particles measured in #/cm3 F. Name: SOA_Average Description: Average number of concentration of aged combustion particles + SOA measured in #/cm3 G. Name: SOA_SD Description: Standard deviation number of concentration of aged combustion particles + SOA measured in #/cm3 Sheet: IL8-A549 1. Number of variables: 12 2. Number of cases/rows:67 3. Variable List A. Name: Date Description: Date in format Month/Date B. Name: FH Description: Fresh combustion particles A549-IL8 inflammatory response concentration fold increase relative to control at the highest dose C. Name: FM Description: Fresh combustion particles A549-IL8 inflammatory response concentration fold increase relative to control at the medium dose D. Name: FL Description: Fresh combustion particles A549-IL8 inflammatory response concentration fold increase relative to control at the lowest dose E. Name: AH Description: Aged combustion particles A549-IL8 inflammatory response concentration fold increase relative to control at the highest dose F. Name: AM Description: Aged combustion particles A549-IL8 inflammatory response concentration fold increase relative to control at the medium dose G. Name: AL Description: Aged combustion particles A549-IL8 inflammatory response concentration fold increase relative to control at the lowest dose H. Name: SH Description: Aged combustion particles + SOA A549-IL8 inflammatory response concentration fold increase relative to control at the highest dose I. Name: SM Description: Aged combustion particles + SOA A549-IL8 inflammatory response concentration fold increase relative to control at the medium dose J. Name: SL Description: Aged combustion particles + SOA A549-IL8 inflammatory response concentration fold increase relative to control at the lowest dose K. Name: NC Description: Negative control A549-IL8 inflammatory response concentration fold increase relative to control at the medium dose L. Name: LPS Description: Positive control (LPS) A549-IL8 inflammatory response concentration fold increase relative to control at the lowest dose Sheet: IL8-A549-THP1 1. Number of variables: 12 2. Number of cases/rows:33 3. Variable List A. Name: Date Description: Date in format Month/Date B. Name: FH Description: Fresh combustion particles A549-THP1-IL8 inflammatory response concentration fold increase relative to control at the highest dose C. Name: FM Description: Fresh combustion particles A549-THP1-IL8 inflammatory response concentration fold increase relative to control at the medium dose D. Name: FL Description: Fresh combustion particles A549-THP1-IL8 inflammatory response concentration fold increase relative to control at the lowest dose E. Name: AH Description: Aged combustion particles A549-THP1-IL8 inflammatory response concentration fold increase relative to control at the highest dose F. Name: AM Description: Aged combustion particles A549-THP1-IL8 inflammatory response concentration fold increase relative to control at the medium dose G. Name: AL Description: Aged combustion particles A549-THP1-IL8 inflammatory response concentration fold increase relative to control at the lowest dose H. Name: SH Description: Aged combustion particles + SOA A549-THP1-IL8 inflammatory response concentration fold increase relative to control at the highest dose I. Name: SM Description: Aged combustion particles + SOA A549-THP1-IL8 inflammatory response concentration fold increase relative to control at the medium dose J. Name: SL Description: Aged combustion particles + SOA A549-THP1-IL8 inflammatory response concentration fold increase relative to control at the lowest dose K. Name: NC Description: Negative control A549-THP1-IL8 inflammatory response concentration fold increase relative to control at the medium dose L. Name: LPS Description: Positive control (LPS) A549-THP1-IL8 inflammatory response concentration fold increase relative to control at the lowest dose Sheet: CYP1A1-A549 1. Number of variables: 9 2. Number of cases/rows:23 3. Variable List A. Name: FH Description: Fresh combustion particles A549 CYP-1A1 induction in (2-ÆÆct) at the highest dose B. Name: FM Description: Fresh combustion particles A549 CYP-1A1 induction in (2-ÆÆct) at the medium dose C. Name: FL Description: Fresh combustion particles A549 CYP-1A1 induction in (2-ÆÆct) at the lowest dose D. Name: AH Description: Aged combustion particles A549 CYP-1A1 induction in (2-ÆÆct) at the highest dose E. Name: AM Description: Aged combustion particles A549 CYP-1A1 induction in (2-ÆÆct) at the medium dose F. Name: AL Description: Aged combustion particles A549 CYP-1A1 induction in (2-ÆÆct) at the lowest dose G. Name: SH Description: Aged combustion particles + SOA CYP-1A1 induction in (2-ÆÆct) at the highest dose H. Name: SM Aged combustion particles + SOA CYP-1A1 induction in (2-ÆÆct)at the medium dose I. Name: SL Description: Aged combustion particles + SOA CYP-1A1 induction in (2-ÆÆct) at the lowest dose Sheet: CYP1A1-A549-THP1 1. Number of variables: 9 2. Number of cases/rows:23 3. Variable List A. Name: FH Description: Fresh combustion particles A549-THP1 CYP-1A1 induction in (2-ÆÆct) at the highest dose B. Name: FM Description: Fresh combustion particles A549-THP1 CYP-1A1 induction in (2-ÆÆct) at the medium dose C. Name: FL Description: Fresh combustion particles A549-THP1 CYP-1A1 induction in (2-ÆÆct) at the lowest dose D. Name: AH Description: Aged combustion particles A549-THP1 CYP-1A1 induction in (2-ÆÆct) at the highest dose E. Name: AM Description: Aged combustion particles A549-THP1 CYP-1A1 induction in (2-ÆÆct) at the medium dose F. Name: AL Description: Aged combustion particles A549-THP1 CYP-1A1 induction in (2-ÆÆct) at the lowest dose G. Name: SH Description: Aged combustion particles + SOA A549-THP1 CYP-1A1 induction in (2-ÆÆct) at the highest dose H. Name: SM Aged combustion particles + SOA A549-THP1 CYP-1A1 induction in (2-ÆÆct) at the medium dose I. Name: SL Description: Aged combustion particles + SOA A549-THP1 CYP-1A1 induction in (2-ÆÆct) at the lowest dose 4. Missing data codes: Code/symbol:blank 5. Specialized formats of other abbreviations used: (2-ÆÆct) is the method of calculating the gene expression of CYP1A1 measured by a polymerase chain reaction (PCR) instrument.