This HuangRestitutionreadme.txt file was generated on 20171012 by Derek Dosdall Links to Publication Field updated. 2021-12-09, BP ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset Data for :Restitution Characteristics of His Bundle and Working Myocardium in Isolated Rabbit Hearts 2. Author Information Principal Investigator Contact Information Name: Derek Dosdall Institution: Nora Eccles Harrison Cardiovascular Research and Training Institute (CVRTI), University of Utah Address: 95 South 2000 East Salt Lake City, Utah 84112 Email: derek.dosdall@utah.edu Associate or Co-investigator Contact Information Name: Shangwei Huang Institution: Department of Cardiology, Shanghai Ruijin Hospital, Shanghai Jiao Tong, University School of Medicine Name: Liqun Huang Institution: Department of Cardiology, Shanghai Ruijin Hospital, Shanghai Jiao Tong, University School of Medicine Name: Nuttanont Panitchob Institution: Nora Eccles Harrison Cardiovascular Research and Training Institute (CVRTI), University of Utah Name: Ravi Ranjan Institution: Nora Eccles Harrison Cardiovascular Research and Training Institute (CVRTI), University of Utah Name: Nan Hu Institution: Department of Internal Medicine, Division of Epidemiology, University of Utah Alternate Contact Information Name: Institution: Address: Email: 3. Date of data collection (single date, range, approximate date) 20160321 to 20160525 4. Geographic location of data collection (where was data collected?): Salt Lake City, UT, USA 5. Information about funding sources that supported the collection of the data: Research reported in this publication was supported by the government of the United States of America, the National Heart, Lung, and Blood Institute (NHLBI) of the National Institutes of Health to DJD under award number R01HL128752. RR is partially supported a NHLBI grant K23HL115084. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: None 2. Links to publications that cite or use the data: Huang, S., Wu, L., Huang, J., Panitchob, N., Hu, N., Ranjan, R., & Dosdall, D. J. (2017). Restitution characteristics of His bundle and working myocardium in isolated rabbit hearts. PloS one, 12(10), e0186880. https://doi.org/10.1371/journal.pone.0186880 3. Links to other publicly accessible locations of the data: 4. Links/relationships to ancillary data sets: None 5. Was data derived from another source? No If yes, list source(s): 6. Recommended citation for the data: --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: Huang Summary data for statistics.xls Short description: spreadsheet of summary data from each cycle length for each animal. Created 20171012 B. Filename: Huang microelectrode data 10 beats.xls Short description: spreadsheet of microelectrode data from 10 beats from each cycle length for every animal. Created 20171012 2. Relationship between files: ‘Huang Summary data for statistics.xls’ is the summary data used in analysis obtained from ‘Huang microelectrode data 10 beats.xls’ 3. Additional related data collected that was not included in the current data package: 4. Are there multiple versions of the dataset? No If yes, list versions: Name of file that was updated: i. Why was the file updated? ii. When was the file updated? Name of file that was updated: i. Why was the file updated? ii. When was the file updated? -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: Microelectrode data was obtained over a wide range of stimulation cycle lengths (CL) in rabbit hearts (n=9) which were isolated, Langendorff- perfused, and electromechanically uncoupled with blebbistatin (10 μM) using standard glass microelectrodes. Pacing pulses were delivered directly to the His bundle. The right atrium was removed to expose the high basal right ventricular septum and the region containing the His bundle. A bipolar electrode was placed on the His bundle for signal discrimination and pacing. Two standard glass microelectrodes (tip resistance, 1 to 5 MΩ, filled with 3 mol/L KCl) were used to record the intracellular action potential (AP) from the His and the right ventricular septal endocardium simultaneously. One microelectrode impaled the His bundle within 2 mm of the bipolar electrode, which was used to verify that the action potential (AP) aligned with the His bundle signal in the bipolar electrode. The other microelectrode recorded the WM signal (Endo group)on the endocardium within 10 mm of the His bundle impalement site. 2. Methods for processing the data: The data were selected if there was a stable microelectrode recording and the last 10 beats of the 30 beats at each pacing CL were analyzed. The APD was measured using LabChart® software at 90% repolarization (APD90). The diastolic interval (DI) was defined as the interval from the end of the repolarization time of the previous beat to the activation time of the next beat. The confirmative analysis focused on comparing if the relationship between APD and DI are the same for the His bundle and working myocardium. We assume the non-linear relationship between APD and DI (ms) 3. Instrument- or software-specific information needed to interpret the data: The signals were acquired by an Axoclamp 900A amplifier (Axon Instruments, USA). All the signals were recorded and monitored in real time using LabChart® software through a Powerlab 16/30 system (AD Instruments, Colorado Springs, CO, USA) 4. Standards and calibration information, if appropriate: 5. Environmental/experimental conditions: 6. Describe any quality-assurance procedures performed on the data: 7. People involved with sample collection, processing, analysis and/or submission: ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: [Huang Summary data for statistics.xls] ----------------------------------------- 1. Number of variables: 5 2. Number of cases/rows: 235 3. Variable List A. Name: [AnimalID] Description: [animal identification number] B. Name: [His1Endo2] Description: [Location of recordings] 1 for His Bundle; 2 for working endocaridum C. Name: [CycleLength] Description: [cycle lengths in milliseconds] 140 to 250ms at 10 ms increments D. Name: [APD] Description: [Action potential duration in milliseconds] E. Name: [DI] Description: [Diastolic interval in milliseconds] ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: [Huang microelectrode data 10 beats.xls] ----------------------------------------- 1. Number of variables: 6 2. Number of cases/rows: 10 3. Variable List A. Name: [RMP] Description: [Resting membrane potential in millivolts] B. Name: [APA] Description: [Action potential amplitude in millivolts] C. Name: [Max dV/dt] Description: [Maximum derivative potential in millivolts] D. Name: [APD30] Description: [Action potential duration at 30% in milliseconds] E. Name: [APD60] Description: [Action potential durations at 60% in milliseconds] F. Name: [APD90] Description: [Action potential durations at 90% in milliseconds]